A novel, precise, accurate and rapid isocratic reversed-phase high performance liquid chromatographic/ultraviolet (RP-HPLC/UV) method was developed, optimized and validated for simultaneous determination of Eperisone hydrochloride and Lornoxicam. The method showed adequate separation for Eperisone hydrochloride and Lornoxicam and best resolution was achieved with Phenomenex analytical C18, (250 mm × 4.6 mm × 5 μm) using methanol–Acetonitrile-water (40:25:35, v/v; pH adjusted to 3.0 with O-phosphoric acid and TEA) as a mobile phase at a flow rate of 1 ml/min and wavelength of 280 nm. The calibration curves were linear over the concentration ranges of 20-70 μg/ml for Eperisone HCl and 1.6-5.6 μg/ml for Lornoxicam. The limit of detection (LOD) and limit of quantification (LOQ) for Eperisone HCl were 1.0467 and 3.1718 μg/ml while for Lornoxicam were 0.0778 and 0.2357 μg/ml, respectively. All the analytes were separated in less than 5.0 min. The proposed method could be applied for routine laboratory analysis of Eperisone hydrochloride and Lornoxicam in pharmaceutical dosage form. Methods were validated statistically and recovery studies were carried out. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in synthetic mixture of both drugs with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.
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